A high content live cell cancer toolbox uniquely enabled by high throughput imaging
By Matthew Boisvert, PhD; Martin Theiss, PhD; Owen Knobb; Lydia Hernandez, PhD
Summary
- Live cell imaging offers a dynamic data-rich assay: here we look at 7 timepoints over 48 hours
- Proliferation, death, nuclear characteristics, cell morphology and mitochondrial phenotype are tracked over time
- We demonstrate dose-dependent cell cycle arrest over time after drug treatment, associating ploidy with cell morphology
- Drug effects on cell and mitochondrial state can be linked to, and separated from, mitotic state on a single cell level
High throughput high content imaging enables quantitative live cell at scale, with subcellular insight
500,000+ cells imaged in ~3 minutes/timepoint in 5 channels, with 0.27µm/pixel digital resolution