Fine actin filopodia and mitochondrial networks in bovine endothelial cells
BPAE cells with mitochondria (red, MitoTracker™ Red CMXRos), actin (green, Alexa Fluor™ 488 Phalloidin), and DNA (blue, DAPI) stains. Source: FluoCells™ slide 1.
Mitochondria visible in distal process of these endothelial cells
BPAE cells with mitochondria (magenta, MitoTracker™ Red CMXRos), actin (cyan, Alexa Fluor™ 488 Phalloidin), and DNA (red, DAPI) stains. Source: FluoCells™ slide 1.
Autophagic vesicles seen throughout cytosol of sarcoma cells after calcium channel blockade
U2OS cells after overnight treatment with 50 µM verapamil, stained for autophagic vesicles (magenta, anti-LC3b IHC), actin (green, phalloidin), and nuclei (blue, Hoechst 33342). Source: in-house assay (see autophagy application note).
Wheat germ agglutinin marks cell membranes in these human epithelial cells
Staining: nuclei (blue, Hoechst 33342), mitochondria (magenta, Mitotracker™ Deep Red FM), and cell membranes (cyan, WGA) stains. Source: in-house assay.
Whole well imaging reveals extent of neural processes in stem cell-derived glutamatergic neurons
7DIV iPSC-derived glutamatergic neurons with nuclei (blue), actin (green, phalloidin), and cytosol (red, CellMask™ Deep Red). Source: Phenovista Biosciences.
Imaging details: 9 min 38 sec to scan full well low volume 384 well plate with 3 fluorescent channels, full resolution, and a focal offset.
Mitochondrial morphology and intensity peaks highlighted in bovine endothelial cells
BPAE cells with mitochondrial dye (grayscale and magenta, MitoTracker™ Red CMXRos). Source: FluoCells™ slide 1.
Brightfield imaging of human bone osteosarcoma (U2OS) cells
Source: in-house assay.
Imaging details: 6 min 31 sec for 96 well plate, maximized well coverage with 3 fluorescent and 1 transmitted light channel, full resolution.
Grayscale and composite images of organelles in bovine endothelial cells
BPAE cells with mitochondria (magenta and green, MitoTracker™ Red CMXRos), actin (cyan and red, Alexa Fluor™ 488 Phalloidin), and DNA (red and blue, DAPI) staining. Source: FluoCells™ slide 1.
20 µm scale in lower right.
Subcellular staining showing diverse morphology of endothelial cells
BPAE cells with mitochondria (magenta and green, MitoTracker™ Red CMXRos), actin (cyan and red, Alexa Fluor™ 488 Phalloidin), and DNA (red and blue, DAPI) staining. Source: FluoCells™ slide 1.
40 µm scale in lower right.
Mitochondrial density highlighted in these osteosarcoma cells
U2OS cells with mitochondria (FireGlo LUT, MitoTracker™ Deep Red) nuclei (blue, Hoechst 33342), actin (green, phalloidin), and cell membranes (cyan, WGA) stains. Source: in-house assay.
Hydroxychloroquine causes autophagic vesicle aggregation in endothelial-like cells
U2OS cells treated with 20 µM hydroxychloroquine overnight. Stained for autophagic vesicles (green, anti-LC3b ICC), actin (magenta, phalloidin), and nuclei (blue, Hoechst 33342). Source: in-house assay (see autophagy application note).
Imaging details: 96 well plate scanned in 3 channels with maximized well coverage 5 min 26 sec.
Autophagy inducer torin-1 degrades autophagic vesicle marker LC3b, resulting in a false negative in this autophagy screen (no autophagic puncta)
U2OS cells treated with 0.125 µM Torin 1 overnight. Stained for nuclei (blue, Hoechst 33342), autophagic vesicles (green, anti-LC3b ICC), and actin (red, phalloidin). Note: Torin 1 targets cytosolic LC3b for degradation. Source: in-house assay.
Imaging details: 96 well plate scanned in 3 channels with maximized well coverage 5 min 26 sec.
Cytoskeletal disruption seen after chemotherapeutic treatment
A549 cells treated with 50 nM paclitaxel overnight and stained for nuclei (cyan, Hoechst 33342), actin (magenta, phalloidin) and microtubules (magenta, anti-alpha-tubulin ICC). Source: in-house assay.