Ultra-Fast Timepoint-Based High Content Imaging Unlocks Live Cell at Scale
Author: Matt Boisvert, PhD., Application Scientist, Araceli Biosciences
Unlock New Insights • Enable the Next Generation of Assay • Scalability Challenges Solved • Minimize Perturbance, Maximize Kinetic Data
This application note explores the transformative capabilities of the Araceli Endeavor® in advancing high-content live cell imaging. For example, >1,000 cells/well can be imaged for an entire 384-well plate in under two minutes, with submicron resolution across four fluorescent channels and brightfield. We discuss the implications of this speed, enabling kinetic assays that avoid fixation artifacts and parse apart subtle phenotypes. We show data from live cells imaged hourly, as well as days apart, in both brightfield and fluorescence. Machine learning-based phenotyping on these cells illustrates the lessened impact of this fast time point based live imaging. This piece also highlights the advantages of brightfield imaging, offering non-perturbating, non-bleaching imaging ideal for AI-driven analysis. Overall, the Endeavor demonstrates unmatched speed, resolution, and scalability—empowering researchers to develop temporally resolved high-content screening workflows with minimal cellular stress.