How can you accurately measure the cellular stress response with single-cell sensitivity yet still be fast enough to analyze a multi-thousand compound library in a day? By visualizing the expression of transcription factor family Nuclear factor-κB (NFκB) and quantifying its post-activation translocation into the nucleus, Araceli Endeavor® high content screening platform paired with our artificial intelligence (AI)-based image analysis software Araceli Voyager demonstrates an effective high-throughput, high-content screening system for cellular stress activation. This application note illustrates the capabilities of Araceli’s high content technology in assaying nuclear translocation, showing a clear effect of cytokine treatment on protein localization from the cytoplasm to nucleus.



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